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anti hsp90  (Proteintech)


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    Structured Review

    Proteintech anti hsp90
    Anti Hsp90, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 672 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+hsp90/HSP90+Antibody/10__1038_slash_s41467___026___78424___w-288-15-16
    Average 96 stars, based on 672 article reviews
    anti hsp90 - by Bioz Stars, 2026-11
    96/100 stars

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    Incubation:

    Article Title: Kynurenine promotes angiogenesis through mTOR signaling in head and neck squamous cell carcinoma.
    Article Snippet: .. Membranes were incubated overnight at 4 °C with primary antibodies, including anti-Hsp90 (1:1000), anti-AhR (1:1000; Protein tech), anti-IDO1, anti-phospho-mTOR, anti-mTOR, anti-phospho-AKT, and anti-AKT (all 1:1000; Cell Signaling Technology), followed by HRP-conjugated secondary antibodies (1:5000, 1 h). ..

    Article Title: ACAT1/SOAT1 governs mitochondrial dynamics and thermogenesis via the mTOR-MFN2-USP2 axis in brown adipocytes
    Article Snippet: Proteins were separated by SDS-PAGE and transferred electrophoretically to a PVDF membrane (Sigma-Aldrich, 24937-79-9). .. Membranes were incubated with primary antibodies overnight at 4 °C: anti-ACAT1 (Purified by our lab), anti-HSP90 (ProteinTech, 60318-1-Ig), anti-Beta Actin (ProteinTech, 66009-1-Ig), antiOXPHOS (Abcam, ab110413), anti-UCP1 (Abcam, ab10983), antiCalnexin (ProteinTech, 10427-2-AP), anti-IP3R (ProteinTech, 19962-1- AP), anti-FACL4 (Abcam, ab155282), anti-VDAC (ProteinTech, 10866-1- AP), anti-Tubulin (ProteinTech, 66031-1-Ig), anti-MFN2 (ProteinTech, 12186-1-AP), anti-MFN1 (ProteinTech, 66776-1-Ig), anti-OPA1 (ProteinTech, 27733-1-AP), anti-DRP1 (ProteinTech, 12957-1-AP), antipSer616-DRP1 (Cell Signaling, 3455), anti-MFF (ProteinTech, 17090-1- AP), anti-Flag (Sigma, F1804), anti-P4D1 (Santa Cruz, sc-8017), anti-HA (ProteinTech, 51064-1-AP), anti-mTOR (ProteinTech, 66888-1-Ig), antipSer2448-mTOR (Cell signaling, 5536), anti-pThr389-p70S6Kinase (Cell signaling, 9234), anti-USP2 (ProteinTech, 10392-1-AP) and antiGNPAT (ProteinTech, 14931-1-AP). .. After incubation with primary antibodies, membranes were washed with TBST and incubated with secondary antibodies conjugated to horseradish peroxidase (HRP; Epizyme, LF101 and LF102) at room temperature for 1 hour.

    Acrylamide Gel Assay:

    Article Title: Energy stress activates AMPK to arrest mitochondria via phosphorylation of TRAK1.
    Article Snippet: .. IP samples were run out on a 6% acrylamide gel and blotted as described above but using anti-HSP90 (60318-1-Ig; Proteintech) as a loading control. .. AMPK arrests mitochondria by phosphorylating TRAK1 https://doi.org/10.1083/jcb.202501023 February 2026 1.8 mL NP-40 lysis buffer (1% NP-40, 15 mM Tris-HCl, pH 7.5, 150 mM NaCl, 1 mM EDTA, pH 8.0, 1 mM dithiothreitol (DTT), 1 mM PMSF, and a protease inhibitor cocktail (11836170001; Roche))

    Article Title: Energy stress activates AMPK to arrest mitochondria via phosphorylation of TRAK1
    Article Snippet: .. IP samples were run out on a 6% acrylamide gel and blotted as described above but using anti-HSP90 (60318-1-Ig; Proteintech) as a loading control. .. For IP of MYC-TRAK2 and MYC-TRAK1 (wild-type and phosphomutants), 3 μg of anti-MYC (SC-40; Santa Cruz) was added to lysates for 1–2 h at 4°C with a rotator.

    Control:

    Article Title: Energy stress activates AMPK to arrest mitochondria via phosphorylation of TRAK1.
    Article Snippet: .. IP samples were run out on a 6% acrylamide gel and blotted as described above but using anti-HSP90 (60318-1-Ig; Proteintech) as a loading control. .. AMPK arrests mitochondria by phosphorylating TRAK1 https://doi.org/10.1083/jcb.202501023 February 2026 1.8 mL NP-40 lysis buffer (1% NP-40, 15 mM Tris-HCl, pH 7.5, 150 mM NaCl, 1 mM EDTA, pH 8.0, 1 mM dithiothreitol (DTT), 1 mM PMSF, and a protease inhibitor cocktail (11836170001; Roche))

    Article Title: Energy stress activates AMPK to arrest mitochondria via phosphorylation of TRAK1
    Article Snippet: .. IP samples were run out on a 6% acrylamide gel and blotted as described above but using anti-HSP90 (60318-1-Ig; Proteintech) as a loading control. .. For IP of MYC-TRAK2 and MYC-TRAK1 (wild-type and phosphomutants), 3 μg of anti-MYC (SC-40; Santa Cruz) was added to lysates for 1–2 h at 4°C with a rotator.

    Bioprocessing:

    Article Title: Role of lipid rafts in the FGFR2c-mediated oncogenic signaling by involvement of TRPA1 channel in pancreatic ductal adenocarcinoma cells.
    Article Snippet: The membranes were incubated with anti-Flotillin (Abcam Cambridge, UK; ab13493) anti-E-cadherin (GT311 GeneTex, Irvine, California, USA), anti-vimentin (M0725, Dako, Glostrup, Denmark), anti-phospho-Fibroblast Growth Factor Receptor Substrate 2 α (FRS2-α) (Tyr196) (Cell Signaling Technology, Beverly, MA, USA, 3864), the anti-phospho-Sarcoma kinase (Src) Family (Tyr416, D49G4) (Cell Signaling Technology, Beverly, MA; USA;6943), monoclonal antibodies anti-pMTOR (Ser 2448; Cell Signaling; Beverly, MA, USA; 5536S), anti-p-p44/42 mitogen-activated protein kinase (MAPK) (p-ERK1/2) (Thr202/Tyr204; Cell Signaling; 9101S), anti-Bek (Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA; C17, sc-122), anti-p-S6K (ser 371, Cell Signaling, Beverly, MA, USA; 9208), and anti p-PKCε (Ser729, Abcam, Cambridge, UK; ab63387), anti-Myeloid Cell Leukemia 1 (Mcl-1) (D35A5) (Cell Signaling; Beverly, MA, USA; 5453), anti-TRPA1 (Abcam; Abcam Cambridge, UK; ab62053),. .. The membranes were stripped as reported [6] and probed again with anti-p44/42 MAPK (ERK1/2) (Cell Signaling; Beverly, MA, USA; 4695S), anti-S6K (Cell Signaling; Beverly, MA, USA;9202), anti-PKCε (Abcam; Abcam Cambridge, UK;ab124806), anti-FRS2 (H-91) (Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA; sc-8318), anti-HSP90 (Proteintech Inc., Rosemont, IL, USA, 13171-1-AP), antiMTOR (Cell Signaling; Beverly, MA, USA; 2983S) polyclonal antibodies or anti-Src (Cell Signaling; Beverly, MA, USA;36D10), anti-ACTB (Sigma-Aldrich; St. Louis, Missouri, USA; A5441) monoclonal antibodies for protein equal loading. ..

    Article Title: Role of lipid rafts in the FGFR2c-mediated oncogenic signaling by involvement of TRPA1 channel in pancreatic ductal adenocarcinoma cells
    Article Snippet: The membranes were incubated with anti-Flotillin (Abcam Cambridge, UK; ab13493) anti-E-cadherin (GT311 GeneTex, Irvine, California, USA), anti-vimentin (M0725, Dako, Glostrup, Denmark), anti-phospho-Fibroblast Growth Factor Receptor Substrate 2 α (FRS2-α) (Tyr196) (Cell Signaling Technology, Beverly, MA, USA, 3864), the anti-phospho-Sarcoma kinase (Src) Family (Tyr416, D49G4) (Cell Signaling Technology, Beverly, MA; USA;6943), monoclonal antibodies anti-p-MTOR (Ser 2448; Cell Signaling; Beverly, MA, USA; 5536S), anti-p-p44/42 mitogen-activated protein kinase (MAPK) (p-ERK1/2) (Thr202/Tyr204; Cell Signaling; 9101S), anti-Bek (Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA; C17, sc-122), anti-p-S6K (ser 371, Cell Signaling, Beverly, MA, USA; 9208), and anti p-PKCε (Ser729, Abcam, Cambridge, UK; ab63387), anti-Myeloid Cell Leukemia 1 (Mcl-1) (D35A5) (Cell Signaling; Beverly, MA, USA; 5453), anti-TRPA1 (Abcam; Abcam Cambridge, UK; ab62053),. .. The membranes were stripped as reported [ ] and probed again with anti-p44/42 MAPK (ERK1/2) (Cell Signaling; Beverly, MA, USA; 4695S), anti-S6K (Cell Signaling; Beverly, MA, USA;9202), anti-PKCε (Abcam; Abcam Cambridge, UK;ab124806), anti-FRS2 (H-91) (Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA; sc-8318), anti-HSP90 (Proteintech Inc., Rosemont, IL, USA, 13171-1-AP), anti-MTOR (Cell Signaling; Beverly, MA, USA; 2983S) polyclonal antibodies or anti-Src (Cell Signaling; Beverly, MA, USA;36D10), anti-ACTB (Sigma-Aldrich; St. Louis, Missouri, USA; A5441) monoclonal antibodies for protein equal loading. ..

    Western Blot:

    Article Title: Decreased Glucose Metabolism and Declined Chaperones Are Unique Features Required for the Survival of Senescent Fibroblasts and Pyruvate Dehydrogenase Is a Potent Senolytic Target
    Article Snippet: .. Antibodies used in the study: anti‐p21 (Cell Signaling Technology, 2947S) for Western blotting and anti‐p21 (Solarbio, K114558P) for immunohistochemistry; anti‐PFKP (Abways, 13389‐1‐AP), anti‐ALDOA (Abways, CY7206), anti‐PKM2 (Immunoway, YM1322); anti‐PDHA (Santa Cruz Biotech, SC‐377092); anti‐GLS1 (CUSABIO, E1218A), anti‐HSP90 (Proteintech, 13171‐1‐AP), anti‐HSP70 (Proteintech, 10995‐1‐AP), anti‐TCP1 (Abways, CY9414), anti‐β‐Actin (Proteintech, 66009‐1‐Ig), and anti‐β‐Tubulin (Proteintech, 10094‐1‐AP). .. Doxorubicin, 2‐deoxy‐glucose (2‐DG), CPI‐613, bis‐2‐(5‐phenylacetamido‐1,3,4‐thiadiazol‐2‐yl)ethyl sulfide (BPTES), and 17‐AAG were purchased from MedChemExpress.

    Immunohistochemistry:

    Article Title: Decreased Glucose Metabolism and Declined Chaperones Are Unique Features Required for the Survival of Senescent Fibroblasts and Pyruvate Dehydrogenase Is a Potent Senolytic Target
    Article Snippet: .. Antibodies used in the study: anti‐p21 (Cell Signaling Technology, 2947S) for Western blotting and anti‐p21 (Solarbio, K114558P) for immunohistochemistry; anti‐PFKP (Abways, 13389‐1‐AP), anti‐ALDOA (Abways, CY7206), anti‐PKM2 (Immunoway, YM1322); anti‐PDHA (Santa Cruz Biotech, SC‐377092); anti‐GLS1 (CUSABIO, E1218A), anti‐HSP90 (Proteintech, 13171‐1‐AP), anti‐HSP70 (Proteintech, 10995‐1‐AP), anti‐TCP1 (Abways, CY9414), anti‐β‐Actin (Proteintech, 66009‐1‐Ig), and anti‐β‐Tubulin (Proteintech, 10094‐1‐AP). .. Doxorubicin, 2‐deoxy‐glucose (2‐DG), CPI‐613, bis‐2‐(5‐phenylacetamido‐1,3,4‐thiadiazol‐2‐yl)ethyl sulfide (BPTES), and 17‐AAG were purchased from MedChemExpress.

    Purification:

    Article Title: ACAT1/SOAT1 governs mitochondrial dynamics and thermogenesis via the mTOR-MFN2-USP2 axis in brown adipocytes
    Article Snippet: Proteins were separated by SDS-PAGE and transferred electrophoretically to a PVDF membrane (Sigma-Aldrich, 24937-79-9). .. Membranes were incubated with primary antibodies overnight at 4 °C: anti-ACAT1 (Purified by our lab), anti-HSP90 (ProteinTech, 60318-1-Ig), anti-Beta Actin (ProteinTech, 66009-1-Ig), antiOXPHOS (Abcam, ab110413), anti-UCP1 (Abcam, ab10983), antiCalnexin (ProteinTech, 10427-2-AP), anti-IP3R (ProteinTech, 19962-1- AP), anti-FACL4 (Abcam, ab155282), anti-VDAC (ProteinTech, 10866-1- AP), anti-Tubulin (ProteinTech, 66031-1-Ig), anti-MFN2 (ProteinTech, 12186-1-AP), anti-MFN1 (ProteinTech, 66776-1-Ig), anti-OPA1 (ProteinTech, 27733-1-AP), anti-DRP1 (ProteinTech, 12957-1-AP), antipSer616-DRP1 (Cell Signaling, 3455), anti-MFF (ProteinTech, 17090-1- AP), anti-Flag (Sigma, F1804), anti-P4D1 (Santa Cruz, sc-8017), anti-HA (ProteinTech, 51064-1-AP), anti-mTOR (ProteinTech, 66888-1-Ig), antipSer2448-mTOR (Cell signaling, 5536), anti-pThr389-p70S6Kinase (Cell signaling, 9234), anti-USP2 (ProteinTech, 10392-1-AP) and antiGNPAT (ProteinTech, 14931-1-AP). .. After incubation with primary antibodies, membranes were washed with TBST and incubated with secondary antibodies conjugated to horseradish peroxidase (HRP; Epizyme, LF101 and LF102) at room temperature for 1 hour.

    Cell-Signaling:

    Article Title: ACAT1/SOAT1 governs mitochondrial dynamics and thermogenesis via the mTOR-MFN2-USP2 axis in brown adipocytes
    Article Snippet: Proteins were separated by SDS-PAGE and transferred electrophoretically to a PVDF membrane (Sigma-Aldrich, 24937-79-9). .. Membranes were incubated with primary antibodies overnight at 4 °C: anti-ACAT1 (Purified by our lab), anti-HSP90 (ProteinTech, 60318-1-Ig), anti-Beta Actin (ProteinTech, 66009-1-Ig), antiOXPHOS (Abcam, ab110413), anti-UCP1 (Abcam, ab10983), antiCalnexin (ProteinTech, 10427-2-AP), anti-IP3R (ProteinTech, 19962-1- AP), anti-FACL4 (Abcam, ab155282), anti-VDAC (ProteinTech, 10866-1- AP), anti-Tubulin (ProteinTech, 66031-1-Ig), anti-MFN2 (ProteinTech, 12186-1-AP), anti-MFN1 (ProteinTech, 66776-1-Ig), anti-OPA1 (ProteinTech, 27733-1-AP), anti-DRP1 (ProteinTech, 12957-1-AP), antipSer616-DRP1 (Cell Signaling, 3455), anti-MFF (ProteinTech, 17090-1- AP), anti-Flag (Sigma, F1804), anti-P4D1 (Santa Cruz, sc-8017), anti-HA (ProteinTech, 51064-1-AP), anti-mTOR (ProteinTech, 66888-1-Ig), antipSer2448-mTOR (Cell signaling, 5536), anti-pThr389-p70S6Kinase (Cell signaling, 9234), anti-USP2 (ProteinTech, 10392-1-AP) and antiGNPAT (ProteinTech, 14931-1-AP). .. After incubation with primary antibodies, membranes were washed with TBST and incubated with secondary antibodies conjugated to horseradish peroxidase (HRP; Epizyme, LF101 and LF102) at room temperature for 1 hour.



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